Immunocytochemical localization of the cystic fibrosis gene product CFTR.

摘要: Antisera against two peptides, corresponding to different domains of the cystic fibrosis gene product CFTR, have been raised and extensively characterized. Both antisera recognize CFTR as a 165-kDa polypeptide in Western analysis cells transfected with cDNA well epithelial cell lines. The tissue distribution has studied by immunocytochemistry. is abundant cells, including those lining sweat ducts, small pancreatic intestinal crypts. Unexpectedly, level lung epithelia relatively low, while it kidney tubules. protein appears be restricted apical, rather than basolateral, regions at least proportion associated plasma membrane. distributions are consistent function for this chloride channel or regulator activity.