Role of CXCL13-CXCR5 Crosstalk Between Malignant Neuroblastoma Cells and Schwannian Stromal Cells in Neuroblastic Tumors

摘要: Neuroblastoma is a stroma-poor (SP) aggressive pediatric cancer belonging to neuroblastic tumors, also including ganglioneuroblastoma and ganglioneuroma, two stroma-rich (SR) less tumors. Our previous gene-expression profiling analysis showed different CXCL13 mRNA expression between SP SR Therefore, we studied 13 tumors by reverse transcription quantitative real-time PCR (RT-qPCR) found that CXCR5b was more expressed in than predominantly Then, isolated Schwannian stromal cells laser capture microdissection malignant neuroblasts express mRNA, whereas CXCL13. Immunohistochemistry confirmed stroma expresses but not CXCR5. To better understand the role of CXCR5 11 neuroblastoma cell lines detected heterogeneous CXCR5b. Interestingly, only splice variant both lines, CXCR5a never detected. Moreover, expressing receptor migrate toward source recombinant Lastly, induced glial differentiation protein. The chemokine released culture medium able stimulate chemotaxis LA1-5S cells. Collectively, our data suggest produced may contribute generation an environment which are retained, thus limiting possible development metastases patients with tumor.