ATP-binding cassette B1 gene polymorphisms, mRNA expression and chemosensitivity to paclitaxel in non-small cell lung cancer cells.

摘要: Background and objective:  The adenosine triphosphate (ATP)-binding cassette, sub-family B, member 1 (ABCB1) gene encodes P-glycoprotein (Pgp), which plays an important role in drug disposition by limiting intracellular uptake of paclitaxel. ABCB1 polymorphisms may alter the expression function Pgp, thereby influencing response to chemotherapy. A panel 17 non-small cell lung cancer (NSCLC) lines was used investigate whether alterations or its mRNA correlated with vitro chemosensitivity paclitaxel. Methods:  Polymorphisms were evaluated direct sequencing. levels assessed quantitative real-time reverse transcription PCR. In paclitaxel expressed as half-maximal inhibitory concentration values, using a tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based colorimetric assay. Results:  variant allele frequencies for four 14.71% 2677G>T/A, 32.35% 2734T>C, 23.53% 3396C>T 76.47% 3435C>T. There significant positive correlation between values (r = 0.5322, P = 0.0279). None associated lines. Conclusions:  These results suggest that high be predictive biomarker poor NSCLC provide clues indications establishing clinically useful relationships given polymorphism level anti-cancer agent.