The pH-Responsive Transcription Factor PacC Governs Pathogenicity and Ochratoxin A Biosynthesis in Aspergillus carbonarius.

摘要: Pathogenic fungi must respond effectively to changes in environmental pH for successful host colonization, virulence and toxin production. Aspergillus carbonarius is a mycotoxigenic pathogen with the ability colonize many plant hosts secrete ochratoxin A (OTA). In this study, we characterized functions addressed role of PacC-mediated signaling A. pathogenicity using designed pacC gene knockout mutant. ΔAcpacC mutant displayed an acidity-mimicking phenotype, which resulted impaired fungal growth at neutral/alkaline pH, accompanied by reduced sporulation conidial germination compared wild type (WT) strain. The was unable efficiently acidify media as direct result diminished gluconic citric acid Furthermore, loss AcpacC complete inhibition OTA production 7.0. Additionally, exhibited attenuated WT toward grapes nectarine fruits. Reintroduction into restored phenotype. Our results demonstrate important roles PacC biosynthesis controlling transcription genes secondary metabolism infection.