Molecular imaging of in vivo calcium ion expression in area postrema of total sleep deprived rats: Implications for cardiovascular regulation by TOF-SIMS analysis

摘要: Abstract Excessive calcium influx in chemosensitive neurons of area postrema (AP) is detrimental for sympathetic activation and participates the disruption cardiovascular activities. Since total sleep deprivation (TSD) a stressful condition known to harm function, present study aimed determine whether vivo expression AP would significantly alter following TSD by use time-of-flight secondary ion mass spectrometry (TOF-SIMS) calretinin (a specific sensor protein neurons) immunohistochemistry. The results indicated that normal rats, intensity was estimated be 0.5 × 105 at m/z 40.08. However, TSD, ions greatly increased 1.2 × 105. Molecular imaging revealed after various strongly expressed signals were distributed throughout with clear identified profiles instead randomly scattered within this region rats. Immunohistochemical staining corresponded well ionic image which majority calcium-enriched gathering co-localized positive neurons. functional significance TSD-induced augmentation demonstrated heart rate mean arterial pressure, clinical markers dysfunction. Considering AP-mediated important regulation, exaggerated render neurocircuitry more vulnerable over-excitation, might serve as underlying mechanism development TSD-relevant deficiency.