Glutamatergic synaptic currents of nigral dopaminergic neurons follow a postnatal developmental sequence.

摘要: The spontaneous activity pattern of adult dopaminergic (DA) neurons the substantia nigra pars compacta (SNc) results from interactions between intrinsic membrane conductances and afferent inputs. In SNc DA neurons, low-frequency tonic background is generated by pacemaker mechanisms, whereas burst generation depends on intact synaptic inputs in particular glutamatergic ones. Tonic release striatum during pacemaking required to maintain motor activity, firing evokes phasic release, necessary for cue-dependent learning tasks. However, it still unknown how properties mature postnatal development before reaching state. We studied developmental profile evoked AMPA NMDA receptor-mediated excitatory postsynaptic currents (EPSCs) brain slices immature (postnatal days P4-10) young (P30-50) tyrosine hydroxylase (TH)-GFP mice. found that somato-dendritic fields are already at P4-10. contrast, EPSCs show a sequence. Spontaneous larger more frequent than ones have bursty pattern. They mediated GluN2B GluN2D subunit-containing receptors. latter generate long-lasting, DQP1105-sensitive, EPSCs, which transiently recorded this early period. Due high large long lasting receptor-dependent (APV-sensitive) bursts response stimulation subthalamic nucleus. conclude transient allows calcium influx into dendrites developing neurons.