Alterations of the cytoskeleton in human cells in space proved by life-cell imaging.

摘要: Microgravity induces changes in the cytoskeleton. This might have an impact on cells and organs of humans space. Unfortunately, studies cytoskeletal microgravity reported so far are obligatorily based analysis fixed exposed to during a parabolic flight campaign (PFC). study focuses development compact fluorescence microscope (FLUMIAS) for fast live-cell imaging under real microgravity. It demonstrates application instrument on-board FTC-133 cancer expressing Lifeact-GFP marker protein visualization F-actin 24(th) DLR PFC TEXUS 52 rocket mission. Although vibration is inevitable part maneuvers, we successfully first time report life-cell cytoskeleton microgravity, gene expression after 31(st) parabola showing clear up-regulation genes. Notably, FLUMIAS reveals significant alterations related Our findings clearly demonstrate applicability rendering it important technological advance when dissecting localization.