GATA-2 regulates granulocyte-macrophage progenitor cell function.
作者： Neil P. Rodrigues , Ashleigh S. Boyd , Cristina Fugazza , Gillian E. May , YanPing Guo
DOI: 10.1182/BLOOD-2008-01-136564
关键词:
摘要: The zinc finger transcription factor GATA-2 has been implicated in the regulation of hematopoietic stem cells. Herein, we explored role as a candidate regulator progenitor cell compartment. We showed that bone marrow from heterozygote (GATA-2+/−) mice displayed attenuated granulocyte-macrophage function colony-forming (CFC) and serial replating CFC assays. This defect was mapped to Lin−CD117+Sca-1−CD34+CD16/32high (GMP) compartment GATA-2+/− marrow, which reduced size functionally impaired assays competitive transplantation. Similar functional impairments were obtained using RNA interference approach stably knockdown wild-type GMP. Although apoptosis cell-cycle distribution remained unperturbed GMP, quiescent cells GMP exhibited altered functionality. Gene expression analysis HES-1 mRNA GATA-2–deficient Binding locus detected myeloid line 32Dcl3, enforced rectified defect, suggesting regulates through HES-1. These data collectively point novel, pivotal determinant fate.
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