Comparison of the ProCyte Dx analyzer with the ADVIA 2120 and the manual differential for validation of equine and bovine hemograms

摘要: The aim of this study was to evaluate the laser-based in-house hematology analyzer ProCyte Dx performing a complete hemogram for bovine and equine blood samples. This is prospective clinically healthy or ill horses (n = 175) cattle (n = 115). EDTA-anticoagulated samples were analyzed within 3–6 h after sampling. Routine variables including differential counts compared reference methods (ADVIA 2120, 200-cell manual leukocyte count). Statistical analysis performed twice, i.e., prior exclusion specimens with graphical reports (dot plots) indicative invalid separation cellular populations. Coefficient variation <3 % (CBC) <7 % (differential count) except eosinophils monocytes (cattle) platelets (PLTs) (horse). Linearity excellent platelets. Spearman's rank correlation coefficients (r s) revealed good s = 0.99–0.80) between both analyzers majority mean corpuscular hemoglobin concentration (MCHC) s = 0.33–0.65), assessed impedance count s = 0.79), (both species). Biases close 0; however, MCHC, (HGB), PLTs large biases seen species. There favorable variables. HGB HGB-derived caused by methodology ADVIA. As other analyzers, correct PLT monocyte are difficult. Dot plot useful tool detect populations thus necessity perform count.