A closed-tube assay for genotyping of the 32-bp deletion polymorphism in the chemokine receptor 5 (CCR5) gene: Dissociation analysis of amplified fragments of DNA
作者： Henrik Rasmussen , Thomas Werge
DOI: 10.1016/J.MCP.2006.05.003
关键词:
摘要: Abstract We have developed a closed-tube assay for determination of the chemokine receptor type 5 (CCR5) 32-bp deletion allele, which protects against infections with HIV and modulates susceptibility to variety inflammatory diseases. This utilizes dissociation analysis amplified products in presence Sybr ® Green I allele discrimination. After having established robust conditions assay, we used it genotype 590 unknown DNA samples. A blinded comparison procedure based upon agarose gel electrophoresis material revealed complete concordance between two procedures. Our is inexpensive easy carry out. Furthermore, reduces or eliminates risk carry-over contamination previously products. The insights gained this study can be applied develop assays genotyping other insertion/deletion polymorphisms differences T m allele-specific amplicons.
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