Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability

摘要: Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most conventional bioassays rely natural antibodies, which are susceptible to elevated temperatures nonaqueous media. Hence, an expensive cumbersome “cold chain” system is necessary preserve the labile antibodies by maintaining optimal cold during transport, storage, handling. Herein, we introduce a facile approach antibody activity biosensor even at temperatures. We show that silk fibroin film could be used protective layer model (Rabbit IgG) cardiac troponin both room temperature 40 °C over several days. Furthermore, simple aqueous rinsing process restores biofunctionality biosensor. This energy-efficient environmenta...