Constitutive synthesis of activator protein 1 transcription factor after viral transformation of mouse fibroblasts.

摘要: Transcription factor activator protein 1 (AP1) interacts with the promoter region of a number genes that are stimulated by growth factors present in serum or agents such as phorbol 12-myristate 13-acetate (PMA) partially mimic their action. To investigate possible role AP1 control cellular and transformation mammalian cells, we monitored its activity binding to specific DNA probe normal transformed mouse NIH 3T3 cells. A 3- 4-fold increase DNA-binding was found after stimulation quiescent 2- 3-fold activation treatment PMA dibutyryl-cAMP, suggesting different signal-transducing pathways could activate these dependent on new synthesis. In contrast high serum-independent cells simian virus 40. These results suggest constitutive synthesis may be crucial step transformation.