Bradykinin increases Na+-K+ pump activity in cultured guinea-pig tracheal smooth muscle cells
作者： Alicia M Dodson , Kerry J Rhoden
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摘要: The effect of bradykinin on the Na+-K+ pump airway smooth muscle was investigated by measuring ouabain-sensitive 86Rb+ uptake in cultured guinea-pig tracheal cells. Bradykinin induced a concentration-dependent increase uptake, with an EC50 3 nM (pD2=8.50±0.10). Stimulation not affected indomethacin (1 μM) suggesting that it is mediated cycloxygenase products arachidonic acid. The B1 receptor agonists Lys-des-Arg9-bradykinin and des-Arg9-bradykinin had no uptake. In contrast, B2 agonist Lys-bradykinin 6 nM (pD2=8.21±0.20). The antagonist des-Arg10-HOE 140 bradykinin-stimulated antagonists HOE WIN 64338 antagonized pKB values (−log M) 8.20±0.08 8.11±0.20 respectively. Reducing extracellular Na+ from 146 mM to 11 mM caused 53.5% decrease basal abolished bradykinin-induced Two inhibitors Na+-H+ exchanger, methylisobutyl-amiloride (MIA; 1–100 μM) ethylisopropyl-amiloride (EIPA; 0.1–10 μM), inhibited without affecting uptake. These results suggest increases activity via stimulation receptors activation exchanger. British Journal Pharmacology (2001) 133, 1339–1345; doi:10.1038/sj.bjp.0704198
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