The approach of using random priming for small forensic DNA-samples

摘要: We are investigating the applicability of a new method called primer-extension-preamplification (PEP; Zhang et al., 1992) for forensic casework. Using standard PCR procedures sensitivity STR’s lies between 50–100 pg, and AmpFLP’s 500 pg — 1ng genomic DNA. In past DNA-samples containing very small amounts template DNA could only be analyzed once. Employing PEP prior to regular we have been able type several VNTR-loci from one PEP-sample. order systematically study advantages disadvantages procedure, amplified DNA-solutions in varying degrees magnitude using random primers aliquots PEP-reaction various VNTR’s.