Dynamic DNA Origami Device for Measuring Compressive Depletion Forces

摘要: The ability to self-assemble nanodevices with programmed structural dynamics that can sense and respond the local environment could enable transformative applications in fields including molecular robotics, nanomanufacturing, nanomedicine. responsive function of biomolecules is often driven by alterations conformational distributions mediated highly sensitive interactions environment. Here, we mimic this approach engineering inherent nanoscale (nanodynamics) into a DNA device exhibits distribution conformations two stable states separated transition state where energy barrier height on scale thermal energy, kBT = 4.1 pN·nm, enabling spontaneous transitions between states. We further establish design principles regulate equilibrium kinetic behavior substituting few strand components. use single-molecule Forster resonance transfer measurements show these nanodynamic properties a...