On the Dynamics of TCR:CD3 Complex Cell Surface Expression and Downmodulation

摘要: TCR downmodulation following ligation by MHC:peptide complexes is considered to be a pivotal event in T cell activation. Here, we analyzed the dynamics of TCR:CD3 surface expression on resting and antigen-activated cells. We show that complex very stable rapidly internalized recycled Surprisingly, internalization rate not increased complexes, despite significant downmodulation, suggesting constitutive rather than ligation-induced serves as force drives serial ligation. Furthermore, mediated intracellular retention ligated degradation lysosomes proteasomes. Thus, our data demonstrate induces preventing recycling inducing internalization.