Comparison of two ASC-derived therapeutics in an in vitro OA model: secretome versus extracellular vesicles.

摘要: In the last years, several clinical trials have proved safety and efficacy of adipose-derived stem/stromal cells (ASC) in contrasting osteoarthritis (OA). Since ASC act mainly through paracrine mechanisms, their secretome (conditioned medium, CM) represents a promising therapeutic alternative. ASC-CM is complex cocktail proteins, nucleic acids, lipids released as soluble factors and/or conveyed into extracellular vesicles (EV). Here, we investigate its potential an vitro model OA. Human articular chondrocytes (CH) were induced towards OA phenotype by 10 ng/ml TNFα presence either or EV, both deriving from 5 × 105 cells, to evaluate effect on hypertrophic, catabolic, inflammatory markers. Given same number donor our data reveal higher compared EV alone that was confirmed enrichment chondroprotective among which TIMP-1 -2 stand out. details, only significantly decreased MMP activity (22% 29% after 3 6 days) PGE2 expression (up 40% at day 6) boosted cytokine. Conversely, treatments down-modulated ~ 30% hypertrophic marker COL10A1. These biological molecular evidences beneficial action CH with may lay basis for future translation cell-free management