High-yield enzymatic glycerolysis of fats and oils

摘要: Several triglyceride fats and oils were reacted with glycerol using lipase as catalyst. A batch system magnetic stirring was used without the addition of any solvents or emulsifiers. In all cases a mixture mono-, di- triglycerides obtained. However, yield monglyceride (MG) depended strongly on reaction temperature: at higher temperatures approximately 30% MG produced equilibrium while lower 65%–90% obtained for most examined. The upper temperature limit below which high could be attained designated critical (Tc). value Tc fat type found to vary between 30°C 46°C naturally occurring hard fats. not fully hydrogenated tallow lard under conditions described here. Of three liquid examined, rapeseed oil olive had 5°C 10°C respectively whereas corn greater. maximum also type: highest yields being (90%), palm stearin milk (80%) lowest (67%). accompanied by solidification mixture. effect enzyme production examined water concentration oil.