Thermal Stability of Low Molecular Weight Urokinase During Heat Treatment. II. Effect of Polymeric Additives
作者： Michael Vrkljan , Thomas M. Foster , Michael E. Powers , Jack Henkin , William R. Porter
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摘要: Turbidimetric or light scattering assays can be used to determine the extent of aggregation in protein formulations. Using low molecular weight urokinase (LMW-UK) as a model protein, effect polymeric additives on heat-induced was evaluated. Previous work has shown that under 60°C heat treatment, LMW-UK initially denatures and unfolded associates form soluble aggregates. Eventually, these aggregates associate precipitate. The effects polymers initial phase examined. Hydroxyethyl (heta) starch, polyethylene glycol 4000, gelatin were found effective, concentration-dependent inhibitors aggregation, whereas polyvinylpyrrolidone (PVP) 300 ineffective. Overall, stability thermally-stressed accounted for by preferential exclusion solute from surface protein.
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