Characterization and Comparison of Protein and Peptide Profiles and their Biological Activities of Improved Common Bean Cultivars (Phaseolus vulgaris L.) from Mexico and Brazil.
作者： Luis Mojica , Elvira González de Mejía , None
DOI: 10.1007/S11130-015-0477-6
关键词:
摘要: Common bean (Phaseolus vulgaris L.) is a good source of protein, vitamins, minerals and complex carbohydrates. The objective was to compare protein profile, including anti-nutrient proteins, potential bioactive peptides improved common cultivars grown in Mexico Brazil. Bean isolates (BPI) were prepared from 15 hydrolyzed using pepsin/pancreatin. Thirteen proteins identified by SDS-PAGE in-gel tryptic-digestion-LC/MS. Protein profile similar among with high concentrations defense-related proteins. Major phaseolin, lectin, protease α-amylase inhibitors. Lectin (159.2 357.9 mg lectin/g BPI), Kunitz trypsin inhibitor (inh) (4.3 75.5 inh/g Bowman-Birk (5.4 14.3 μg trypsin-chymotrypsin BPI) activity (2.5 14.9 % inhibition relative acarbose/mg higher Mexican beans compared Brazilian beans. Abundant HPLC-MS/MS molecular masses ranging 300 1500 Da significant sequences SGAM, DSSG, LLAH, YVAT, EPTE KPKL. Potential bioactivities sequenced angiotensin converting enzyme (ACE), dipeptidyl peptidase IV (DPP-IV) antioxidant capacity. Peptides presented biological activities related control hypertension type-2 diabetes.
springer.com
sci-hub.se 参考文章(23)
Luis Mojica, Karen Chen, Elvira González de Mejía, None, Impact of commercial precooking of common bean (Phaseolus vulgaris) on the generation of peptides, after pepsin-pancreatin hydrolysis, capable to inhibit dipeptidyl peptidase-IV. Journal of Food Science. ,vol. 80, ,(2015) , 10.1111/1750-3841.12726
Lidia López-Barrios, Janet A. Gutiérrez-Uribe, Sergio O. Serna-Saldívar, Bioactive peptides and hydrolysates from pulses and their potential use as functional ingredients. Journal of Food Science. ,vol. 79, ,(2014) , 10.1111/1750-3841.12365
Ana Angelica Feregrino-Perez, Carmen Piñol-Felis, Xavier Gomez-Arbones, Ramón G Guevara-González, Rocio Campos-Vega, Jorge Acosta-Gallegos, Guadalupe Loarca-Piña, None, A Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis Plant Foods for Human Nutrition. ,vol. 69, pp. 248- 254 ,(2014) , 10.1007/S11130-014-0428-7
Cecilia Silva-Sánchez, AP Barba De La Rosa, M Fabiola León-Galván, Ben O de Lumen, Antonio de León-Rodríguez, E González De Mejía, None, Bioactive peptides in amaranth (Amaranthus hypochondriacus) seed. Journal of Agricultural and Food Chemistry. ,vol. 56, pp. 1233- 1240 ,(2008) , 10.1021/JF072911Z
Diego A Luna-Vital, Luis Mojica, Elvira González de Mejía, Sandra Mendoza, Guadalupe Loarca-Piña, None, Biological potential of protein hydrolysates and peptides from common bean (Phaseolus vulgaris L.): A review Food Research International. ,vol. 76, pp. 39- 50 ,(2015) , 10.1016/J.FOODRES.2014.11.024
Marta Alves, Inês Chaves, Dina Carrilho, Manuela Veloso, Cândido Pinto Ricardo, Detection of novel trypsin inhibitors in the cotyledons of Phaseolus vulgaris seeds. Journal of Plant Physiology. ,vol. 167, pp. 848- 854 ,(2010) , 10.1016/J.JPLPH.2010.01.007
Bahareh H. Sarmadi, Amin Ismail, Antioxidative peptides from food proteins: a review Peptides. ,vol. 31, pp. 1949- 1956 ,(2010) , 10.1016/J.PEPTIDES.2010.06.020
Miguel E Oseguera-Toledo, Elvira Gonzalez De Mejia, Vermont P Dia, Silvia L Amaya-Llano, None, Common bean (Phaseolus vulgaris L.) hydrolysates inhibit inflammation in LPS-induced macrophages through suppression of NF-κB pathways Food Chemistry. ,vol. 127, pp. 1175- 1185 ,(2011) , 10.1016/J.FOODCHEM.2011.01.121
Thaís de Souza Rocha, Luis Manuel Real Hernandez, Yoon Kil Chang, Elvira González de Mejía, None, Impact of germination and enzymatic hydrolysis of cowpea bean (Vigna unguiculata) on the generation of peptides capable of inhibiting dipeptidyl peptidase IV Food Research International. ,vol. 64, pp. 799- 809 ,(2014) , 10.1016/J.FOODRES.2014.08.016
Carlos A. Montoya, Jean-Paul Lallès, Stephen Beebe, Pascal Leterme, Phaseolin diversity as a possible strategy to improve the nutritional value of common beans (Phaseolus vulgaris) Food Research International. ,vol. 43, pp. 443- 449 ,(2010) , 10.1016/J.FOODRES.2009.09.040