A specific drug targeting system based on polyhydroxyalkanoate granule binding protein PhaP fused with targeted cell ligands.

摘要: Abstract Polyhydroxyalkanoates (PHA) is a family of intracellular biopolyesters produced by many bacteria. PHA granule binding protein PhaP able to bind hydrophobic polymers via strong interaction. A receptor-mediated drug delivery system was developed in this study based on PhaP. The consists nanoparticles, and polypeptide or ligands fused nanoparticles were used package mostly drugs; with over-expression their corresponding genes Pichia pastoris , E. coli attach nanoparticle. At the end, pull PhaP–PHA targeted cells receptors recognized ligands. It found that specific ligand–PhaP–PHA taken up macrophages, hepatocellular carcinoma cell BEL7402 vitro liver, vivo, respectively, when mannosylated human α1-acid glycoprotein (hAGP) epidermal growth factor (hEGF), which macrophages cells. nanoparticle clearly visible organs (liver tumor) under fluorescence microscopy rhodamine B isothiocyanate (RBITC) as model due targeting effect created ligand receptor binding. hEGF–PhaP–nanoparticles carrying RBITC be endocytosed tumor tumorous mice. Thus, proven effective both vivo .