Determination of cefmenoxime in human body fluids by high-performance liquid chromatography.

摘要: A rapid and reliable method for the quantitative determination of cefmenoxime in serum urine by reversed phase high-performance liquid chromatography is described. Serum was deproteinized with acetonitrile. Urine diluted dilute acetic acid (17.5 mmol/l). Separations were performed isocratic mode using a C18 type column precolumn packed Perisorb RP/8. The eluant consisted mixture acetonitrile 25.0 mmol/l ratio 32/69 (vol/vol). In normal subjects well separated from endogenous compounds various added drugs. Its complete separation confirmed selective degradation beta-lactamase fromBacillus cereus UV spectrophotometry. detection limit 0.3 mg/l at wave-length 254 nm. Peak areas gave linear results up to concentrations 500 mg/l. Within-batch precision (coefficient variation) ranged 1.1 6.2 %. Recovery rates varied 99.0 103.3%. Results standard microbiological assay correlated those obtained present HPLC method. Eight healthy volunteers who given single intravenous dose 1 g excreted 86.3 ± 5.8 % unchanged drug within 24 h urine.