Hospital routine analysis of penicillins, third-generation cephalosporins and aztreonam by conventional and high-speed high-performance liquid chromatography.

摘要: Abstract A high-performance liquid chromatographic procedure for the measurement of fifteen β-lactam antibiotics in body fluids is described, with special reference to high-speed techniques. The involves a unique sample preparation before analysis all following compounds: benzyl-penicillin, ampicillin, cloxacillin, ticarcillin, mezlocillin, azlocillin, piperacillin, cefotaxine and its desacetyl metabolite, cefsulofin, cefoperazone, cefmenoxime, ceftazidime, ceftriaxone monobactam aztreonam; thus biological samples arriving at laboratory can be treated batch. Of these antibiotics, eleven chromatographed same type mobile phase, which consists mixture ammonium acetate acetonitrile various ratios. Three others need ion-pairing chromatography because their polarity, ticarcillin requires citric acid. High-speed seems particularly suitable routine columns equilibrate more rapidly, retention times are much shorter, detection limits lower longer lifetime reduces costs.