Induction of sterile transcription from the kappa L chain gene locus in V(D)J recombinase-deficient progenitor B cells.

摘要: B cell development in RAG-2-deficient (RAG-2T) mice is impeded at an early stage, due to the inability of these animals rearrange their endogenous ig gene loci. Expression E mu-bcl-2 transgene did not change this phenotype. However, stromal cell/IL-7-reactive progenitors (pro-B cells) were found fetal live and bone marrow RAG-2T RAG-2T/E transgenic numbers comparable normal mice. Like cells from they are c-kit+, surrogate L chain+ CD25-, can proliferate vitro for long periods time. Upon IL-7 deprivation, be induced differentiate into c-kit-, chain- CD25+ that no longer clonable on IL-7. Furthermore, sterile transcription kappa chain loci induced. The latter was also observed with pro-B directly isolated ex vivo animals. results suggest progenitor differentiation occur V(D)J recombinase-deficient stage where kL rearrangements would normally initiated. It further indicates some molecular programs take place absence Ig rearrangements.