Isolation and structural characterization of the rat acyl-CoA oxidase gene.

摘要: Abstract Overlapping genomic clones containing the entire sequence of rat acyl-CoA oxidase gene were isolated and characterized. This spans about 25 kilobases, there are 14 exons 13 introns. All exon-intron junction sequences agree with GT/AG rule. The results Southern blot analyses indicated that occurs once per haploid genome. In a preceding paper (Miyazawa, S., Hayashi, H., Hijikata, M., Ishii, N., Furuta, Kagamiyama, Osumi, T., Hashimoto, T. (1987) J. Biol. Chem. 262, 8131-8137), we described presence two species mRNA which have different sequences, only in small region. We identified corresponding to this above produced by alternative splicing these exons. Several cap sites detected S1 mapping primer extension method. principal one was mapped at 75 nucleotides upstream initiator methionine codon. 5'-flanking region sequenced up 1300 nucleotides, sites. There neither "TATA" box nor typical "CCAAT" sequence, least nucleotide -500, rather, is G + C-rich (65%). "GC" hexanucleotides (GGGCGG or CCGCCC) repeat six times Two them, located nearest sites, complete 11-base pair direct repeat, 5'-GGGCGGGGCCG-3'. features resemble those for enoyl-CoA hydratase:3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme. possible functional significance characteristic elements transcriptional regulation given attention.