Multiple mRNAs for 3-hydroxy-3-methylglutaryl coenzyme A reductase determined by multiple transcription initiation sites and intron splicing sites in the 5'-untranslated region.

摘要: The current studies show that mRNAs with 16 different 5'-untranslated regions (varying in length from 68 to 670 nucleotides) are produced the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene hamster UT-1 cells. This complex pattern of results a combination multiple transcription initiation sites and 5' splice donor for intron region gene. Analysis was made possible by modification S1 nuclease technique which we used series progressively truncated uniformly labeled, single-stranded [32P]DNA probes addition usual end-labeled 32P-probes. All diminished when cells incubated sterols, indicating all them subject coordinate control.