In vitro maturation, fertilization, embryo development & clinical outcome of human metaphase-I oocytes retrieved from stimulated intracytoplasmic sperm injection cycles.

摘要: Background & objectives: The major cause of fertilisation failure after ICSI is the oocyte to initiate biochemical processes necessary for activation. This inability could be ascribed cytoplasmic immaturity those gametes even if they had reached nuclear maturity. activation a mature characterised by release from metaphase II (MII) arrest and extrusion second polar body, followed pro-nuclear formation. aim this study was evaluate fate in vitro matured (IVM) I (MI) oocytes subjected intracytoplasmic sperm injection (ICSI) at different time intervals first body (1PB) fertilization (IVF) cycles. Methods: A total 8030 were collected 1400 cycles, 5504 MII cumulus retrieval. Four hundred eight (27.1%) culture 2-26 h 5389 sibling moment denudation injected. On other hand, 49 cycles containing only MI retrieval injected three reaching MII. as follows: 2-6 (n=10), 8-11 (n=4) 23-26 (n=10). Fertilization development potential evaluated both studies. Results: Fertilization, embryo cleavage quality significantly lower IVM compared denudation. Pregnancy rate higher group achieved transfers when performed within (group I) II) PB extrusion. One pregnancy obtained healthy neonate born. Interpretation conclusions: Immature women whose ovaries have been stimulated matured, fertilized ICSI, cleaved give rise live birth. However, developmental competence embryos derived immature reduced, with vivo oocytes. Further, human need between 2-6h 1PB complete its maturation.