Brushing the surface: cascade reactions between immobilized nanoreactors

摘要: Functionalization of hard or soft surfaces with, for example, ligands, enzymes proteins, is an effective and practical methodology the development new applications. We report assembly two types nanoreactors based upon poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) diblock copolymers as scaffold, uricase lactoperoxidase bio-catalysts located within nanoreactors, melittin biopores inserted into hydrophobic shell. The were immobilized on poly(2-hydroxyethyl methacrylate)-co-poly(2-aminoethyl methacrylate hydrochloride) (PHEMA-co-P(2-AEMA·HCl) brushes-grafted wafer by utilizing strong supramolecular interactions between biotin streptavidin. brushes silicon prepared a surface initiating atom transfer radical polymerization (ATRP) “graft-from” technique. Cascade reactions different surface-anchored demonstrated converting Amplex® Red to fluorescent probe resorufin using H2O2 produced from uric acid H2O. detailed properties functionalized including binding behaviours cascade investigated emission spectroscopy, transmission electron microscopy (TEM), light scattering (LS), atomic force (AFM) quartz crystal microbalance (QCM-D). results are proof-of-principle preparation catalytically functional engineered materials lay foundation applying this advanced material in biosensing, implanting antimicrobial preparation.