Transition Metals and Organic Ligands Influence Biodegradation of 1,4-Dioxane

摘要: 1,4-Dioxane, a contaminant increasingly detected in water supplies, is public health concern because it classified as possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals cofactors their catalytic sites, may toxic at elevated concentrations. In this study, the effects of transition on 1,4-dioxane biodegradation Pseudonocardia dioxanivorans CB1190, monooxygenase-expressing bacterium, were investigated. Dose-dependent inhibition Cd(II), Cu(II), and Ni(II) was observed, whereas Zn(II) had no measurable effect rates. cultures exposed to 2 mg/L Cu(II) restored presence 0.005, 0.05, 0.5 mM alginin, cysteine, 0.005 mM tannin. These results indicated that specific ligands bind with alleviate bacterial toxicity. parallel experiments, tannin cysteine inhibited biodegradation, but BSA, SRNOM did not affect Thus, rates are subject interactions among natural organic environment. Mechanistic insights quantitative data obtained study will useful for designing bioremediation strategies sites simultaneously contaminated pollutants.