Response and recovery of microbial communities subjected to oxidative and biological treatments of 1,4-dioxane and co-contaminants.

摘要: Microbial community dynamics were characterized following combined oxidation and biodegradation treatment trains for mixtures of 1,4-dioxane chlorinated volatile organic compounds (CVOCs) in laboratory microcosms. Bioremediation is generally inhibited by co-contaminate CVOCs; with only a few specific bacterial taxa reported to metabolize or cometabolize being unaffected. Chemical hydrogen peroxide (H2O2) as non-selective demonstrated 50-80% removal regardless the initial CVOC concentrations. Post-oxidation bioaugmentation metabolizer Pseudonocardia dioxanivorans CB1190 removed remaining 1,4-dioxane. The intrinsic microbial population, biodiversity, richness, biomarker gene abundances decreased immediately after brief phase, but recovery cultivable microbiomes more diverse observed during subsequent 9-week phase. Results generated from Illumina Miseq sequencing bioinformatics analyses established that oxidative stress tolerant genus Ralstonia was abundant step, Cupriavidus, Pseudolabrys, Afipia, Sphingomonas identified dominant genera aerobic incubation. Multidimensional analysis elucidated separation populations function time under all conditions, suggesting temporal succession determining factor independent CVOCs mixtures. Network highlighted potential interspecies competition commensalism, line shifts predominant various developing directions different steps train. Collectively, this study chemical followed effective treating 1,4-dioxane, even presence high levels residual peroxide, disinfectant, enhanced our understanding ecological impacts These results will be valuable predicting synergies lead cost savings improved remedial outcomes short-term active remediation well long-term changes environmental communities.