EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging

摘要: Objectives Colorectal cancer initially lies dormant as dysplasia, a premalignant state that provides an opportunity for early detection. Dysplasia can be flat in morphology, focal size, and patchy distribution, thus it appears "invisible" on conventional wide-field endoscopy. Aims We aim to develop validate peptide is specific epidermal growth factor receptor (EGFR), cell surface target overexpressed colonic adenomas readily accessible imaging. Methods expressed purified the extracellular domain of EGFR use with phage display identify QRHKPRE binds 2 this target. A near-infrared fluorescence endoscope was used perform vivo imaging binding spontaneous mouse model topical administration. also validated human immunohistochemistry immunofluorescence. Results After labeling Cy5.5, we knockdown competition studies. Peptide cells occurred within 2.46 min had affinity 50 nm. No downstream signaling observed. measured target-to-background ratio 4.0±1.7 2.7±0.7, polyps lesions, respectively. On immunofluorescence specimens, greater intensity from dysplasia than normal found 19.4-fold difference. Conclusions have selected contrast agent overexpress