Monocyte chemoattractant protein-1 is sufficient for the chemotaxis of monocytes and lymphocytes in transgenic mice but requires an additional stimulus for inflammatory activation.

摘要: Monocyte chemoattractant protein-1 (MCP-1), a chemotactic cytokine, acts in vitro as and activating factor for multiple types of leukocytes. To determine the effects MCP-1 vivo, we constructed transgenic mice that express human type II alveolar epithelial cells secrete it into bronchoalveolar space. We found overexpression led to marked increase numbers both monocytes lymphocytes could be recovered by lavage. This accumulation mononuclear leukocytes reversed administration an MCP-1-blocking Ab. In spite its effect, expression did not cause inflammatory activation accumulated Lungs also showed no morphologic evidence inflammation. However, had increased sensitivity other stimuli. treated with either i.p. LPS or i.v. yeast wall glucan developed consolidated pulmonary infiltrates consisting predominantly macrophages. Nontransgenic such infiltrates. These results demonstrate is vivo alone does cells, but leads enhanced response upon treatment