In silico modeling of the interaction between TEX19 and LIRE1, and analysis of TEX19 gene missense SNPs.
作者： Faisal A Alzahrani , Yousef MohammedRabaa Hawsawi , Hisham N Altayeb , Naif O Alsiwiehri , Othman R Alzahrani
DOI: 10.1002/MGG3.1707
关键词:
摘要: Background Testis expressed 19 (TEX19) is a specific human stem cell gene identified as cancer-testis antigen (CTA), which emerged potential therapeutic drug target. TEX19.1, mouse paralog of TEX19, can interact with LINE-1 retrotransposable element ORF1 protein (LIRE1) and subsequently restrict mobilization elements in the genome. Aim This study aimed to predict interaction TEX19 LIRE1 analyze missense polymorphisms. model was generated using I-TASSER between studied HADDOCK software. Methods The stability docking formed complex through molecular dynamic simulation GROMACS. Missense SNPs (n=102) were screened for their effects on structure function different Results Outcomes this revealed amino acids that potentially stabilize predicted interface LIRE1. Of these SNPs, 37 play probably damaging role protein, three them (F35S, P61R, E55L) located at binding site could disturb affinity. Conclusion information be verified by further vitro vivo experimentations exploited targets.
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