The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate

摘要: Li Y-C, Kuan Y-H, Huang F-M, Chang Y-C. The role of DNA damage and caspase activation in cytotoxicity genotoxicity macrophages induced by bisphenol-A-glycidyldimethacrylate. International Endodontic Journal, 45, 499–507, 2012. Abstract Aim  To evaluate the potential toxicological implications BisGMA on murine macrophage cell line RAW264.7. Methodology  Lactate dehydrogenase release, flow cytometry, Western blot fluorometric assays were used to detect viability, mode death activities, respectively. In addition, alkaline single-cell gel electrophoresis cytokinesis-block micronucleus applied genotoxicity. Statistical analyses performed using anova followed Bonferroni’s t-test for multi-group comparisons test. Results  demonstrated a cytotoxic effect RAW264.7 cells dose-dependent time-dependent manner (P < 0.05). was found induce two modes death. changed from apoptosis necrosis as concentrations elevated. Caspase-3, caspase-8 caspase-9 activities significantly Moreover, exhibited via dose-related increase numbers strand breaks (P < 0.05). Conclusions  Cytotoxicity are mediated activation.