Tissue factor activity and ECM-related gene expression in human aortic endothelial cells grown on electrospun biohybrid scaffolds.

摘要: All blood vessels are lined with a quiescent endothelium, which aids in regulating regular flow and avoiding thrombus formation. Current attempts at replacing diseased frequently fail due to the intrinsic thrombogenicity of materials used as vascular grafts. In extending our previous work where we introduced new candidate scaffolds for grafts electrospun from blend solution PLGA, gelatin, elastin (PGE), this study aimed evaluate potential PGE support nonthrombogenic monolayers primary isolates human aortic endothelial cells (HAECs), assessed by combination biochemical, molecular, bioinformatics-based analyses. After 24 h culture on 3-D fibrous scaffolds, HAECs formed confluent, nonthrombogenic, physiologically competent monolayer, tissue factor (TF) gene expression protein activity assays. The levels TF mRNA/protein grown were similar those gelatin or collagen IV-coated 2-D surfaces. addition, analysis focused microarray containing 84 ECM-related cDNA probes demonstrated that essentially expressed histotypic "transcriptome" more than cultured coverslips coated (a well-known "inert" substrate conventional EC culture), but less so films. These data suggest an important role nanorough substrates (PGE films) passivating confirm crucial effect composition process. Principal component above (including IV) implied plays greater surface topography affecting "transcriptome". Taken together, findings potentially suitable application small diameter engineering.