Nanofiber Alignment Regulates NIH3T3 Cell Orientation and Cytoskeletal Gene Expression on Electrospun PCL+Gelatin Nanofibers.

摘要: To examine the influence of substrate topology on behavior fibroblasts, tissue engineering scaffolds were electrospun from polycaprolactone (PCL) and a blend PCL gelatin (PCL+Gel) to produce matrices with both random aligned nanofibrous orientations. The addition scaffold was shown increase hydrophilicity matrix proliferation NIH3T3 cells compared alone. orientation nanofibers within did not have an effect adherent cells, but substrates elongate align parallel direction fiber alignment. A microarray cyotoskeleton regulators probed differences in gene expression between grown randomly oriented substrates. It found that transcriptional eight genes statistically different two conditions, all them being upregulated condition. proteins encoded by these are linked production polymerization actin microfilaments, as well focal adhesion assembly. Taken together, data indicates fibroblasts themselves their related genes.