A specific and sensitive antigen capture assay for NS1 protein quantitation in Japanese encephalitis virus infection

摘要: Abstract Japanese encephalitis virus (JEV) is a human pathogenic, mosquito-borne flavivirus that endemic/epidemic in Asia. JEV rarely detected or isolated from blood cerebrospinal fluid (CSF), and detection of IgM generally diagnostic the infection. The nonstructural glycoprotein NS1 released transiently during replication. aim this study was to set up quantitative antigen capture assay. A soluble hexameric form protein produced stable Drosophila S2 cell clone purified supernatant fluids. Two IgG1 monoclonal antibodies (MAbs) with high affinity against two different epitopes were used develop an antigen-capture assay limit 0.2 ng ml −1 NS1. Up 1 μg ml supernatants mammalian cells infected but sera virus-infected mice before onset death. Moreover, at low levels ( ) 23.8% 10.5% CSF patients diagnosed as IgM-positive for JEV. This test proposed highly specific diagnosis acute infection genotypes I IV.