Immunohistochemical and In Situ Hybridization Analysis of Steroidogenic Enzymes for Study of Steroid Metabolism in Endocrine Organs

摘要: Recent advancements in radioimmunoassays, enzyme-linked immunoassays, and a variety of chromatography techniques have made it possible to measure circulating steroid hormones with reasonable accuracy. However, obtain better understanding metabolism is important analyze the tissues that synthesize secrete steroids. Major steroid-producing organs, including adrenal cortex, ovary, testis, are all associated complicated morphological features. Therefore essential determine localization steroidogenesis, i.e., produced specific cells tissue. Biochemical studies tissue concentration steroids activity steroidogenic enzymes, certainly provided information. owing nature invitro biochemical analysis, nearly impossible which parts capability. efforts been directed toward analysis steroidogenesis morphologically sections.