MicroRNA-4458 suppresses the proliferation of human lung cancer cells in vitro by directly targeting Lin28B
作者： Chang-hong Liu , De-sheng Lv , Mo Li , Ge Sun , Xue-fei Zhang
DOI: 10.1038/APS.2017.73
关键词:
摘要: Previous studies have shown that the expression of microRNA-4458 (miR-4458) is dysregulated in hepatocellular carcinoma and colon cancer. In this study, we investigated direct target miR-4458 its biological functions human lung cancer cells. By using database TargetScan, identified Lin28B, an oncogene, as a gene miR-4458. dual-luciferase reporter assay, found mimics dose-dependently inhibited luciferase activity wild-type 3′UTR Lin28B A549 NCI-H1299 cell lines without affecting mutant forms, whereas anti-miR-4458, inhibitor miR-4458, promoted forms. Overexpression significantly decreased protein levels cells, growth colony formation. Conversely, knockdown with anti-miR-4458 increased proliferation, which could be reverted by expression. addition, detected RT-PCR 40 tissues matched peritumoral tissues, was overexpressed negatively correlated (r=-0.694, P<0.05). We conclude tumor suppressor, These results suggest modulation miR-4458/Lin28B offers potential therapeutic strategy for
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