Epigenetic analyses of planarian stem cells demonstrate conservation of bivalent histone modifications in animal stem cells.
作者： Anish Dattani , Damian Kao , Yuliana Mihaylova , Prasad Abnave , Samantha Hughes
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摘要: Planarian flatworms have an indefinite capacity to regenerate missing or damaged body parts owing a population of pluripotent adult stems cells called neoblasts (NBs). Currently, little is known about the importance epigenetic status NBs and how histone modifications regulate homeostasis cellular differentiation. We developed improved optimized ChIP-seq protocol for in Schmidtea mediterranea generated genome-wide profiles active marks H3K4me3 H3K36me3, suppressive H3K4me1 H3K27me3. The these were found correlate well with NB gene expression profiles. that genes transcriptional activity compartment but which switch on post-mitotic progeny during differentiation are bivalent, being marked by both H3K27me3 at promoter regions. In further support this hypothesis, bivalent also high level paused RNA Polymerase II promoter-proximal region. Overall, study confirms control important maintenance program makes case promoters as conserved feature animal stem not vertebrate-specific innovation. By establishing robust analysis methodology, we promote planarians promising model system investigate modification-mediated regulation cell function
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