Interspecific cross of Brassica oleracea var. alboglabra and B. napus : effects of growth condition and silique age on the efficiency of hybrid production, and inheritance of erucic acid in the self-pollinated backcross generation

摘要: Interspecific hybrids were produced from reciprocal crosses between Brassica napus (2n = 38, AACC) and B. oleracea var. alboglabra 18, CC) to introgress the zero-erucic acid alleles into oleracea. The ovule culture embryo rescue technique was applied for production of F1 plants. effects silique age, as measured by days after pollination (DAP), growth condition (temperature) on efficiency this investigated. greatest numbers per under 20°/15°C (day/night) at 16 DAP (♀) × crosses, while 15°/10°C 14 crosses. Application also increased BC1 (F1 oleracea) hybrid 10-fold over in vivo seed set. segregation erucic self-pollinated backcross generation, i.e. BC1S1 seeds, revealed that gametes plants carrying a greater number A-genome chromosomes more viable. This resulted significantly intermediate smaller high-erucic seeds.