Sequencing and expression of the rne gene of Escherichia coli

摘要: RNase E is a major endonucleolytic RNA processing enzyme in Escherichia coli. We have sequenced 3.2 kb EcoRI-BamHI fragment encoding the rne gene, and identified its reading frame. Upstream from there are appropriate consensus sequences for putative promoter ribosome binding site. translated this gene using T7 polymerase/promoter system. determined 25 amino acids N-terminal of product they full agreement with DNA sequence. The migrates SDS containing polyacrylamide gels as 110,000 Da polypeptide, but open frame found indicates much smaller protein. entity that contains RNA, which could account, at least partially, migration gels.