A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum.

摘要: A synthetic promoter library (SPL) for Lactobacillus plantarum has been developed, which generalizes the approach obtaining promoters. The consensus sequence, derived from rRNA promoters extracted L. WCFS1 genome, was kept constant, and non-consensus sequences were randomized. Construction of SPL performed in a vector (pSIP409) previously developed high-level, inducible gene expression sakei. wide range strengths obtained with approach, covering 3–4 logs levels small increments activity. evaluated ability to drive β-glucuronidase (GusA) aminopeptidase N (PepN) expression. Protein production constitutive, most potent gave high protein comparable those native promoters, PepN corresponding approximately 10–15 % total cellular protein. High correlation between activities when tested sakei plantarum, indicates potential other species. enables fine-tuning stable various applications plantarum.