Retinal pigment epithelium damage enhances expression of chemoattractants and migration of bone marrow-derived stem cells

摘要: PURPOSE: To characterize chemoattractants expressed by the retinal pigment epithelium (RPE) after sodium iodate (NaIO3)-induced damage and to investigate whether ocular-committed stem cells preexist in bone marrow (BM) migrate response chemoattractive signals damaged RPE. METHODS: C57/BL6 mice were treated with a single intravenous injection of NaIO3 (50 mg/kg) create RPE damage. At different time points real-time RT-PCR, ELISA, immunohistochemistry used identify secreted subretinal space. Conditioned medium from NaIO3-treated mouse was an vitro assay assess chemotaxis cell antigen-1 positive (Sca-1+) BM mononuclear (MNCs). The expression early ocular markers (MITF, Pax-6, Six-3, Otx) migrated MNCs isolated granulocyte colony-stimulating factor (G-CSF) Flt3 ligand (FL)-mobilized nonmobilized peripheral blood (PB) analyzed RT-PCR. RESULTS: mRNA for stromal cell-derived factor-1 (SDF-1), C3, hepatocyte growth (HGF), leukemia inhibitory (LIF) significantly increased, higher SDF-1 C3 protein secretion found treatment. A number BMMNCs expressing conditioned retina. There also increased PBMNCs mobilization. CONCLUSIONS: Damaged secretes cytokines that have been shown serve as BM-derived (BMSCs). Retina-committed appear reside can be mobilized into PB G-CSF FL. These may potential endogenous source tissue regeneration