作者: Deborah Leckband
DOI: 10.1146/ANNUREV.BIOPHYS.29.1.1
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摘要: ■ Abstract Although the force fields and interaction energies that control protein behavior can be inferred indirectly from equilibrium kinetic measurements, recent developments have made it possible to quantify directly (a) ranges, magnitudes, time dependence of forces between biological materials; (b) mechanical properties isolated proteins; ( c) strength single receptor- ligand bonds. This review describes results obtained by using atomic microscope, optical tweezers, surface apparatus, micropipette aspiration short-range protein-ligand interactions long-range, nonspecific together behavior. The examples presented illustrate power measurements