Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase

摘要: The distribution of the U3 small nuclear RNA during cell cycle CHO line was studied by in situ hybridization using digoxigenin-labelled oligonucleotide probes. location hybrids immunofluorescence microscopy and at ultrastructural level correlated with two nucleolar proteins, nucleolin fibrillarin. snRNA molecules persist throughout mitosis close association remnant. is present prenucleolar bodies (PNBs) could participate nucleologenesis several proteins such as interaction snRNP 5′ external spacer pre-RNA newly synthesized active NORs proposed to be promoting event nucleologenesis.