Physicochemical Characteristics of Patent Blue Violet Dye

摘要: Physicochemical data for patent blue violet dye (I) are reported. The pKa protonation of the first diethylanilino group was 2.78 ± 0.03. absorptivity values calculated a 1% (w/v) solution previously dried I at pH 7.4 were 1650, 170, and 250 638, 412, 309 nm, respectively. A table wavelength maxima observed color as function Ho five spectra certain included. chemistry is explained, scheme showing its two protonated carbonium ions triphenylcarbinol derivative presented. distribution coefficients in n-octanol or chloroform phosphate buffer systems 0.013 0.12, approximate solubilities 25° six organic solvents solubility analysis distilled water Results latter suggest that forms lyotropic mesophase high aqueous concentrations. Compound poorly lipid soluble. Samples 1.000% 0.9% NaCl, formulated with without (v/v) benzyl alcohol autoclaving, varied not more than 5% from initial content during storage dark under constant fluorescent light 25 5° 20 months. Data TLC several eluents indicated degree purity dye. half-lives Joss 5 × 10-4% solutions potassium hydroxide 13.7, 12.7, 11.3, 10.0 1.2 hr, 17.0 9.5 days, 180 fraction bound to 4% human serum albumin 37° ranged 0.05 0.83, corresponding unbound postdialysis concentration range 1.7 10-4 2.0% (w/v). Scatchard plot binding revealed one high-affinity site, K = 6235 M-1 low-affinity sites, average affinity constants 33 M-1. support fact spectrophotometric determination 639 2 nm appears comprise stability-indicating assay.