Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering

作者: Kornelia Jumel , Immo Fiebrig , Stephen E. Harding

DOI: 10.1016/0141-8130(95)01071-8

关键词:

摘要: The weight average molar masses and mass distributions of two commercial pig gastric mucins fresh mucin preparations were determined by the technique size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both samples exhibited much lower averages than freshly prepared material contained more impurities. On other hand, a preparation examined after being stored frozen for 18 months revealed slight increase in mass.

参考文章(23)
I.A. Nieduszynski, Dynamic properties of biomolecular assemblies International Journal of Biological Macromolecules. ,vol. 12, pp. 335- ,(1990) , 10.1016/0141-8130(90)90024-5
L.A. Bobek, H Tsai, A.R. Biesbrock, M.J. Levine, Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7). Journal of Biological Chemistry. ,vol. 268, pp. 20563- 20569 ,(1993) , 10.1016/S0021-9258(20)80762-5
F. A. Meyer, A. Silberberg, Structure and function of mucus. Ciba Foundation Symposium 54 - Respiratory Tract Mucus. pp. 203- 218 ,(1982) , 10.1002/9780470720356.CH10
Charles Tanford, Physical Chemistry of Macromolecules ,(1961)
W. Banks, C.T. Greenwood, J. Sloss, Light-scattering studies on aqueous solutions of amylose and amylopectin Carbohydrate Research. ,vol. 11, pp. 399- 406 ,(1969) , 10.1016/S0008-6215(00)80582-0
Abigail Ball, Stephen E. Harding, John R. Mitchell, Combined low speed sedimentation equilibrium/gel permeation chromatography approach to molecular weight distribution analysis: application to a sodium alginate International Journal of Biological Macromolecules. ,vol. 10, pp. 259- 264 ,(1988) , 10.1016/0141-8130(88)90001-3
P. Johnson, Light scattering in the study of colloidal and macromolecular systems International Reviews in Physical Chemistry. ,vol. 12, pp. 61- 87 ,(1993) , 10.1080/01442359309353278