Influence of Activin A Supplementation During Human Embryonic Stem Cell Derivation on Germ Cell Differentiation Potential

摘要: Human embryonic stem cells (hESCs) are more similar to “primed” mouse epiblast (mEpiSCs). mEpiSCs, which derived in Activin A, show an increased propensity form primordial germ cell (PGC)-like response bone morphogenic protein 4 (BMP4). Hence, we hypothesized that hESCs the presence of A may be competent differentiating towards PGC-like after supplementation with BMP4 compared standard hESC lines. We were able successfully derive two lines pluripotent and showed higher base levels STELLA cKIT without addition. Furthermore, upon differentiation as embryoid bodies BMP4, observed upregulation VASA at day 7, both transcript level lines, appeared take longer time for PGC specification. Unlike other nuclear pSMAD2/3 confirmed signalling was switched on A-derived They also responsive based detection pSMAD1/5/8 endodermal a result GATA-6 expression. our results provide novel insights into impact derivation its subsequent influence potential vitro.