MicroRNA in sperm from Duroc, Landrace and Yorkshire boars.

摘要: Sperm contain microRNAs (miRNAs), which may have roles in epigenetic control. Regarding phylogenetic relationships among various swine breeds, Yorkshire and Landrace, are considered phenotypically genetically very similar, but distinctly different from Duroc. The objective of the present study was to compare abundance boar sperm miRNAs these three breeds. Overall, 252 prioritized were investigated using real-time PCR; relative expression similar Landrace boars, significantly compared Seventeen (hsa-miR-196a-5p, hsa-miR-514a-3p, hsa-miR-938, hsa-miR-372-3p, hsa-miR-558, hsa-miR-579-3p, hsa-miR-595, hsa-miR-648, hsa-miR-524-3p, hsa-miR-512-3p, hsa-miR-429, hsa-miR-639, hsa-miR-551a, hsa-miR-624-5p, hsa-miR-585-3p, hsa-miR-508-3p hsa-miR-626) down-regulated (P < 0.05; fold regulation ≤−2) sperm, Duroc sperm. Furthermore, (hsa-miR-9-5p, hsa-miR-150-5p, hsa-miR-99a-5p) up-regulated However, 240 not (within + 2 fold) between We concluded that there significant differences those two breeds boars. integrated target genes for selected (identified via an in-silico method) appeared participate spermatogenesis functions.